Skip to content

The evidence

Why spinach?

The first question scientists ask us. Here is the published research, what we have measured, and what the field trials will test.

published sources
16published sources
spinach compounds
6spinach compounds
field trials
Nextfield trials
Glycine betaine20-HydroxyecdysoneDefensinsOxalic acidAmino acidsPhytochemicals

Three levels of evidence

Plant extracts are a recognised class of crop biostimulant. This is where RootMatrix stands on each level.

  1. Published

    What each compound does

    16 peer-reviewed papers and US EPA rulings, linked below.

  2. Seen in our lab

    What RootMatrix did

    • No burn at field strength (1 seed test)
    • Stays put in water, redisperses
    • Stable at pH 7, 6 months sealed
    • 20 L batches reproduced
  3. Next

    What spinach adds in the field

    Replicated trials with research partners: yield, grain zinc, nitrogen uptake and N₂O.

Job one

Spinach builds the mineral

Leaf compounds keep the mineral fine and stable while it forms, in place of synthetic reagents like EDTA.

13 nm

even, hexagonal zinc particles grown with spinach extract as the stabiliser

Our honest note: these studies made nanoparticles, not our layered mineral. RootMatrix applies the same principle, and the process is patent-pending.

Job two

Spinach carries compounds with a paper trail

Glycine betaineStress shield

>100 µmol/g

dry leaf, stored by spinach's plant family under salt stress

Applied to plants, glycine betaine raises their tolerance to abiotic stress such as salt, drought and temperature.

Not claimed yet: How much glycine betaine each litre of RootMatrix carries.

20-HydroxyecdysoneNematode moulting disruptor

4.2×10⁻⁷ M

the level above which it disrupted plant-parasitic nematodes

Root-knot, cyst and lesion nematodes moulted abnormally, invaded roots less and died. Spinach is one of very few crops that makes it in quantity.

Not claimed yet: Whether a field dose reaches that level in the root zone.

DefensinsAntimicrobial peptides

<20 µM

active against bacterial wilt, bacterial canker and Fusarium fungi

Spinach defensins made inside citrus trees gave tolerance to citrus greening. The US EPA has exempted their residues in citrus from a food tolerance.

Not claimed yet: Whether the peptides stay active after formulation.

Oxalic acidPhosphorus unlocker

~1,000 µmol/L

the oxalate level that freed bound phosphate in soil

Root acids release phosphorus held by soil minerals. Of citrate, malate and oxalate, oxalate freed the most phosphate.

Not claimed yet: A dose that high in the field. We count it as a root-zone bonus, not a phosphorus claim.

Amino acidsNatural chelation

Amino-chelates

nutrients bound to amino acids such as glycine and glutamate

Described as safer and more efficient than routine fertiliser salts or synthetic EDTA chelates, especially as sprays.

Not claimed yet: The same review notes that crop-response data are still limited.

Why it matters

One leaf, several jobs.

Zinc sulphate and EDTA chelates bring minerals only. Spinach adds stress, pest and soil-chemistry compounds with published modes of action.

See the trial design
Water onlyRootMatrix 1:20
Illustration of what we saw. Not drawn to a measured scale.

Our own data, so far

Our root test, in the open

Seed
Green gram (Vigna radiata)
Length
5 days
Control
Water only
RootMatrix
1 ml in 20 ml, the field dilution

What it shows

  • No burn: seeds sat in RootMatrix for 5 days
  • Roots looked longer and thicker
  • Leaves more open, sprouting more even

What it doesn't show yet

  • How much longer, in millimetres
  • That it repeats across dishes
  • What spinach adds on its own

Next: 7 days, with numbers

The repeat that measures it

  1. AWaterthe control
  2. BRootMatrix 1:20repeats the first test
  3. CRootMatrix 1:10strongest label rate, for burn
  4. DSpinach extract alonewhat spinach adds
  5. EZinc and magnesium sulphatesame zinc and magnesium dose
  6. FRootMatrix without spinachif a batch can be made

4 coded dishes × 20 seeds per arm. Germination, root and shoot length, burn score, germination index and vigour index.

Field trials, next

The trial that answers “why spinach?”

Four arms, side by side. Only the fourth has both the mineral and spinach.

  1. Arm 1

    Farmer practice

    The usual NPK and urea

  2. Arm 2

    Same nutrients from salts

    Zn, Mg and S at the RootMatrix dose

  3. Arm 3

    Spinach extract alone

    Isolates what spinach adds

  4. Arm 4

    RootMatrix

    Mineral and spinach together

To measure in every arm

  • Yield
  • Grain zinc
  • Nitrogen uptake
  • Soil N₂O
  • Nematode counts
  • Disease score

To test in every batch

Glycine betaine, 20-hydroxyecdysone and oxalate content, tested at an accredited lab, so the dose reaching the field is known.

The papers

Every source opens at its DOI or official record. Checked on PubMed, Crossref and the US Federal Register, September 2026.

  1. 1du Jardin 2015du Jardin (2015), Scientia Horticulturae 196:3–14: plant biostimulants, definition, concept, main categories and regulation
  2. 2Ahmad et al. 2022Ahmad, Blasco & Martos (2022), Frontiers in Plant Science 13:862034: combating salinity through natural plant extract biostimulants, a review
  3. 3Djouadi & Derouiche 2021Djouadi & Derouiche (2021), Current Research in Green and Sustainable Chemistry 4:100214: spinach-mediated synthesis of zinc oxide nanoparticles
  4. 4Abert et al. 2022Abert, Aduwamai & Adamu (2022), Biochemistry Research International 2022:9311227: iron oxide nanoparticles green-synthesised with spinach extract
  5. 5Chen & Murata 2008Chen & Murata (2008), Trends in Plant Science 13:499–505: glycinebetaine, an effective protectant against abiotic stress in plants
  6. 6Weretilnyk et al. 1989Weretilnyk, Bednarek, McCue, Rhodes & Hanson (1989), Planta 178:342–352: Chenopodiaceae accumulate over 100 µmol glycine betaine per g dry leaf when salinised
  7. 7Weretilnyk & Hanson 1990Weretilnyk & Hanson (1990), PNAS 87:2745–2749: betaine-aldehyde dehydrogenase cloned from salt-stressed spinach
  8. 8Soriano et al. 2004Soriano, Riley, Potter & Bowers (2004), Journal of Chemical Ecology 30:1885–1899: phytoecdysteroids, a novel defense against plant-parasitic nematodes
  9. 9Bakrim et al. 2008Bakrim, Maria, Sayah, Lafont & Takvorian (2008), Plant Physiology and Biochemistry 46:844–854: ecdysteroids in spinach
  10. 10Segura et al. 1998Segura, Moreno, Molina & García-Olmedo (1998), FEBS Letters 435:159–162: novel defensin subfamily from spinach
  11. 11Padilla et al. 2025Padilla et al. (2025), Plant Biotechnology Journal 23:1876–1878: naturally occurring spinach defensins confer tolerance to citrus greening and potato zebra chip
  12. 12US EPA 2021US EPA, Federal Register 2021-18786 (14 Sep 2021): spinach defensin proteins in citrus, temporary exemption from the requirement of a tolerance
  13. 13US EPA 2026US EPA, Federal Register 2026-06686 (7 Apr 2026): citrus tristeza virus expressing spinach defensins SoD2, final rule exempting citrus residues from a tolerance
  14. 14Jones 1998Jones (1998), Plant and Soil 205:25–44: organic acids in the rhizosphere, a critical review
  15. 15Kukreja et al. 2025Kukreja, Noriega Landa, Lee, Engle & Darrouzet-Nardi (2025), Biogeochemistry 169:4: organic acid thresholds to mobilise phosphorus in dryland soils
  16. 16Souri 2016Souri (2016), Open Agriculture 1:118–123: aminochelate fertilizers, the new approach to the old problem

A question we haven't answered?

Ask the founder directly. Scientists get a straight answer, data or not.